96-well PCR procedure

IMPORTANT: Use the Clear everything command before beginning the 96-well procedure. In the screen shots below, the Clear everything command was given before the DNA files and the primers files were opened.

For selected cases (e.g. HIV, SARS, influenza), PCR can be run in a 96-well plate, and viral load values determined.  For example, for Case A of SARS, open the DNA and primer files, click on the primer file, then shift-click to select the DNA files as shown below.  Then use the Quick Load / Run button at the bottom of the Opened & Processed window to make the menu selections as shown below.

Once the plate is loaded as indicated by the orange wells, click the Run button as shown below…

After reading the results on the plate, use the Options button and make the menu selections as shown below to determine viral load for the various samples…

…and the viral load values appear.  If desired, use the Options button again to restore the original text.