Tutorials

GETTING STARTED WITH CASE IT V7

Follow the instructions on the two PDF documents below before using Case It v7:

How to run Case It on both Windows and Mac OSX

Installing MEGA bioinformatics software for use with Case It (optional, but recommended)


TUTORIALS SPECIFIC TO CASE IT V7

Quantitative PCR (qPCR) (video, PDF, Powerpoint)

Bioinformatics analyses using MABL, MAFFT, and MEGA (PDF, Powerpoint)*

*Note: The Aligning sequences/tree building video tutorial (also linked below) was created using an earlier version of the Case It simulation (Case It v6.06), which only used MEGA software for bioinformatics analyses.  This tutorial would work for Case It v7 as well, if you are using MEGA rather than the the MABL or MAFFT web sites to align sequences and build trees.


VIDEO TUTORIALS FOR BOTH CASE IT V6 AND CASE IT V7

Video tutorials below use were created using Case It v6, but are suitable for use with Case It v7.  (They can also be accessed from the Tutorials menu of Case It v7.)

SNP microarray SNP background, loading and running SNP microarray cases, connecting to online databases, calculations for genotype calling
Expression microarray Background information, loading and running expression microarray cases, connecting to online databases for interpreting results
Aligning sequences/tree building Accessing MEGA software from within Case It, to align sequences and build trees
ELISA and Western blot Using auto-loading features to quickly load protein and antibody samples
Dot blot (autoload) Open and load DNA and probe files quickly
Southern blot (autoload) Select DNA and probe samples and get Southern blot results
Differential display feature Demonstrates the Differential Display feature used to visually determine the relative amount of DNA in a given band, in Southern blots and gels
PCR, digest DNA and run gels on Data screen Select samples and get quick gel results with autoload features
Show PCR primer sites Shows forward and reverse primer sites, with an animation of the first step of the PCR process for any sequence
Digest DNA and run gels on the Lab bench Use simulated tools to set up restriction enzyme digest, load and run gel