Features of Case It version 6 and version history

• 7/26/12 – an issue was fixed with the Sequence Analysis window.

• 3/13/12 @2:43 CT – Updated version released that fixed a bug causing the ELISA window to change shape when it was moved.

• 2/8/12 @6:45 pm CT – Updated version released that fixed several issues, including labels not appearing in some fields when an ELISA or dot blot was loaded. This was a labeling issue only, and did not otherwise affect the accuracy of results. The Differential Display feature for DNA gels and blots was also updated

• 1/26/12 – Case It v6.06 released – first beta version.

• 12/17/10: Case It v6.05 has a useful new feature that allows for DNA gel bands to be narrower, to better distinguish between band patterns. Small buttons appear above the gel when a lane is loaded, enabling the width of the bands to be changed, and disappear when the gel is cleared. This feature is not found in Case It v6.04.

• 12/17/10: Case It v6.05 allows for DNA files containing more than 990 sequences to be loaded into gel wells. Only the first 990 sequences can be displayed as bands on these gels, however.

• Case It v6 does not take up the entire screen, since the main window is now moveable. Dragging the main window moves all dependent windows, keeping the same relative positions of all dependent windows, even after they are moved. This allows for a separate browser window to be visible for tutorials, access to problem spaces, etc.

• Export features have been built into the ‘Opened & processed’ window and also a new ‘Sequence analysis’ window, for the purpose of bioinformatics analyses.

• Case It v6 exports sequences for multiple alignment via CLUSTALW (included with the Case It download), and then displays trees and alignments via MEGA5 (a separate, free download).

• Problem spaces provide access to sequences for research applications

• The enhanced ‘Opened & processed window’ includes new menu commands for automatic loading and running of laboratory procedures, decreasing the time needed to set up these procedures (it is still possible to load and run manually, however). Auto-loading/running is now possible for DNA and protein electrophoresis, PCR (including multiplex and 96-well PCR), blotting, and ELISA.

• The new ‘Sequence analysis’ window has enhanced capabilities for the analysis of DNA and protein sequences, including: resizable fields for (1) returning search results, (2) compiling sequences for export, (3) and search / replace.

• The number of fragments possible per lane has been increased from 40 to over 900.

• The number of sequences, primers, probes and enzymes that can be open simultaneously has been increased from 60 to 200 (300 for DNA and protein sequences).

• Multiple sequences in a single file will open as separate lines in the Opened & processed windows, as long as the sequences are in FASTA format (i.e. the first line of each separate sequence of the file is a definition line, beginning with the character >.)